Cell-Free Translation Is More Variable than Transcription
نویسندگان
چکیده
منابع مشابه
Cell-Free Translation Is More Variable than Transcription.
Although RNA synthesis can be reliably controlled with different T7 transcriptional promoters during cell-free gene expression with the PURE system, protein synthesis remains largely unaffected. To better control protein levels, we investigated a series of ribosome binding sites (RBSs). Although RBS strength did strongly affect protein synthesis, the RBS sequence could explain less than half of...
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ژورنال
عنوان ژورنال: ACS Synthetic Biology
سال: 2017
ISSN: 2161-5063,2161-5063
DOI: 10.1021/acssynbio.6b00250